Welcome to TH lab
The synthesis of proteins using genetic information is a fundamental process in all life forms. In higher organisms, the process is tightly controlled and much of this regulation occurs during the initial steps of translation. Translational control plays an important role in many key life processes, including early embryonic development, learning and memory, and the response to cellular stress. Although translation initiation is a fundamental and indispensable process, many of its aspects are poorly understood. We employ biochemical, mutational, and structural biology approaches to understand the molecular details of the initial steps of protein synthesis and to figure out how it is regulated. Understanding translation initiation and its regulation will be beneficial for many human disorders and cancers. In addition, it may provide avenues for the development of strategies for modulation of translation initiation and development of novel therapeutic strategies against bacterial, fungal, and viral infection.
Peer-reviewed Research publications (from IISc):
1. Basu I, Gorai B, Chandran T, Maiti PK# and Hussain T,# Selection of start codon during mRNA scanning in eukaryotic translation initiation Commun Biol 5, 587 (2022); doi.org/10.1038/s42003-022-03534-2
2. M Afsar, R Narayan, MN Akhtar, Das D, H Rahil, Nagaraj SK, SM Eswarappa, S Tripathi, Hussain T#. Drug targeting Nsp1-ribosomal complex shows antiviral activity against SARS-CoV-2. eLife (2022);11:e74877 doi: 10.7554/eLife.74877
3. Llácer JL*#, Hussain T*#, Dong J*, Villamayor L, Gordiyenko Y, Hinnebusch AG# (2021) Large-scale movement of eIF3 domains during translation initiation modulate start codon selection. Nucleic Acids Res. (2021) Oct 14:gkab908. doi: 10.1093/nar/gkab908. Online ahead of print.
4. Kumar R, Afsar M, Khandelwal N, Chander Y, Riyesh T, Dedar RK, Gulati BR, Pal Y, Barua S, Tripathi BN#, Hussain T#, Kumar N#. Emetine suppresses SARS-CoV-2 replication by inhibiting interaction of viral mRNA with eIF4E. Antiviral Res. (2021):189,105056. doi: 10.1016/j.antiviral.2021.105056.
5. Datta M, Pillai M, Modak MJ, Liiv A, Khaja FT, Hussain T, Remme J, Varshney U. A mutation in the ribosomal protein uS12 reveals novel functions of its universally conserved PNSA loop. Mol Microbiol. (2020): doi: 10.1111/mmi.14675.
6. Mishra RK, Datey A, Hussain T#. mRNA recruiting eIF4 factors involved in protein synthesis and its regulation. Biochemistry (2020): 59, 34-46. doi: 10.1021/acs.biochem.9b00788.
7. Poornima G, Mythili R, Nag P, Parbin S, Verma PK, Hussain T. and Rajyaguru PI. RGG-motif self-association regulates eIF4G-binding translation repressor protein Scd6. RNA Biol. (2019):16(9):1215-1227. doi: 10.1080/15476286.2019.1621623.
8. Llácer JL*, Hussain T*, Saini AK*, Nanda J*, Kaur S, Gordiyenko Y, Kumar R, Hinnebusch AG, Lorsch JR, and Ramakrishnan V. Translational initiation factor eIF5 replaces eIF1 on the 40S ribosomal subunit to promote start-codon recognition. eLife (2018): 7:e39273.
Manuscript submitted/under review:
1. Singh J, Mishra RK, Ayyub SA, Hussain T, Varshney U, The initiation factor 3 (IF3) residues interacting with the initiator tRNA elbow modulate the fidelity of the translation initiation and growth fitness in Escherichia coli. (under revision in Nucleic Acids Res.)
Other publications (Editorial):
1. Dutta, S.#, Hussain, T.# Editorial. J Indian Inst Sci 98, 229–230 (2018). https://doi.org/10.1007/s41745-018-0089-x
( #Corresponding Author * Equal Contribution)
Peer-reviewed Research publications (At the time of joining IISc)
8. Hussain T*, Llacer JL*, Wimberly B*, Jeffrey S. Kieft and Ramakrishnan V. Large-Scale Movements of IF3 and tRNA during Bacterial Translation Initiation. Cell (2016) 167: 133-144.
9. Llacer JL*, Hussain T*, Marler L, Aitken CE, Thakur A, Lorsch JR, Hinnebusch AG and Ramakrishnan V. Conformational differences between open and closed states of the eukaryotic translation initiation complex. Mol Cell (2015) 59: 399-412.
10. Ahmad S, Muthukumar S, Kuncha SK, Routh SB, Yerabham AS, Hussain T, Kamarthapu V, Kruparani SP, Sankaranarayanan R. Specificity and catalysis hardwired at the RNA-protein interface in a translational proofreading enzyme; Nat Commun. (2015) Jun 26;6:7552. doi: 10.1038/ncomms8552.
11. Hussain T*, Llacer JL*, Fernandez IS, Munoz A, Martin-Marcos P, Savva CG, Lorsch JR, Hinnebusch AG and Ramakrishnan V. Structural changes enable start codon recognition by the eukaryotic translation initiation complex; Cell (2014) 159: 597-607.
12. Amunts A*, Brown A*, Bai XC*, Llácer JL*, Hussain T, Emsley P, Long F, Murshudov G, Scheres SH, Ramakrishnan V. Structure of the yeast mitochondrial large ribosomal subunit; Science (2014) 343(6178): 1485-1489.
13. Ahmad S*, Routh SB*, Kamarthapu V, Chalissery S, Sowndarya M, Hussain T, Krupani SP, Deshmukh MV, Sankaranarayanan R. Mechanism of chiral proofreading during translation of the genetic code; eLife (2013) 2: e01519.
14. Fernández IS*, Bai XC*, Hussain T, Kelley AC, Lorsch JR, Ramakrishnan V, Scheres SH. Molecular architecture of a eukaryotic translational initiation complex; Science. (2013) 342(6160): 1240585.
15. Matharu NK, Hussain T, Sankaranarayanan R, Mishra RK. Vertebrate homologue of Drosophila GAGA factor; J. Mol. Biol. (2010) 400, 434-447.
16. Hussain T, Kamarthapu V, Kruparani SP, Deshmukh MV, Sankaranarayanan R. Mechanistic insights into cognate substrate discrimination during proofreading in translation; Proc Natl Acad Sci USA (2010) 107, 22117–22121.
17. Hussain T*, Kruparani SP*, Pal B, Dock-Bregeon AC, Dwivedi S, Shekar MR, Sureshbabu K, Sankaranarayanan R. Post-transfer editing mechanism of a D-aminoacyl-tRNA deacylase-like domain in threonyl-tRNA synthetase from archaea; EMBO J. (2006) 25, 4152–4162.(* Equal Contribution)
Postdoctoral research - MRC Laboratory of Molecular Biology, Cambridge, UK
Ph.D - Centre for Cellular and Molecular Biology, Hyderabad, INDIA
DBT/Wellcome Trust India Alliance Intermediate Fellowship
INSA (Indian National Science Academy) Young Scientist Medal 2012
EMBO (European Molecular Biology Organization) Long Term Fellowship 2011
Career Development Fellowship, 2011 by MRC Laboratory of Molecular Biology, Cambridge, UK
First Prize in Eli Lilly and Company Asia Outstanding Thesis Award, 2011
David Blow Scholarship 2009 by CCP4 UK
Ranbaxy Science Foundation Young Scientist Award, Medical Sciences, 2006-07
Dr. K. V. Rao Annual Research Award, Biological Sciences, 2006-07
Year of joining: 2017
Research topic- Role of eIF4 factors in mRNA recruitment to the ribosome
M.Sc. in Biotechnology - MSU Baroda
B.Sc. - Nagpur University
Year of joining: 2017
Research topic- Delivery of initiator tRNA to the ribosome by non-canonical initiation factors
M.Phil- Utkal University
M.Sc- Ravenshaw University
B.Sc.- Utkal University
Rishi Kumar Mishra
Year of joining: 2017 (Integrated Ph.D)
Research topic- Gaining structural insights of eIF4F complex involved in translation initiation
M.S - Indian Institute of Science, Bangalore
B.Sc. Honours in Botany - Banaras Hindu University (2013-16)
Year of joining: 2019
Research topic- Investigating the crosstalk among initiation factors in IRES-mediated translation initiation
M.Sc in Biotechnology- Savitribai Phule Pune University (2017 - 19)
B.Sc in Microbiology (honours)- Scottish Church College, University of Calcutta (2014 - 17)
Year of joining : 2019
Research topic- Understanding the mechanism of non-canonical translation initiation
M.Sc Microbiology, Savitribai Phule Pune University (2017-19)
B.Sc. Microbiology, M.J college, KBC North Maharashtra University Jalgaon (2014-17)
Year of Joining: 2020 (Integrated PhD)
Research topic- Exploring structural aspects of RNA helicases involved in translation initiation
M.S: Indian Institute of Sciences (2019-2021)
B.Sc: Biotechnology (Ram Niranjan Jhunjhunwala College University of Mumbai) (2017-2019)
Vivek Singh Pal
Year of joining: 2020
Research topic- Exploring delivery of tRNA by non-canonical means to the ribosome.
M.Sc in Botany, Banaras Hindu University (2018- 20)
B.Sc in Botany Honours, Banaras Hindu University (2015-18)
Year of joining: 2022
Research topic- Gaining insights into the structural aspects of translation initiation machinery in prokaryotes
BS-MS dual degree (Major in Biological Sciences) from IISER Berhampur, Odisha (2016 - 2021)
Year of joining: 2022 (Integrated Ph.D)
M.Sc : Ongoing in Life Sciences at Indian Institute of Science, Bangalore
B.Sc. in Biotechnology, Adamas University , Kolkata (2019-2022)
Post doc and project fellows
Postdoctoral research fellow
Year of joining- 2022
Ph.D- Shoolini University (2021)
Integrated M.Sc in Zoology- Shoolini University (2012-17)
Project Associate II
Year of joining- 2022
JRF- National Centre for Cell Sciences, Pune (2019-21)
M.Sc in Biotechnology- Banaras Hindu University (2016-18)
B.Sc in Biotechnology Honours- Mumbai University (2013-16)
Current UG students
1. Anjaney Pandey
1. Sruti Patel
1. Dr. Thyageshwar Chandran, (Post TH lab: Assistant Professor NIT Warangal, India)
2. Dr. Jyoti Gupta (Post TH lab : Post-doc fellow at University of Florida, Scripps biomedical research, USA)
3. Dr. Mohammad Afsar (Post TH lab: post-doc fellow at UT Health, San Antonio, USA)
4. Dr. Faizal Tarique Khaja (Post TH lab: post-doc at UT Health, San Antonio, USA)
1. Agnismita Mondal
2. Priyabrata Nag (2017-2020)
3. Huma Rahil (2020-2021)
1. Rohit Sunil (2019-21)
2. Adwaith B Uday (2019-2022)
3. Mohit Das (2021-2022)
4. Ahmed PK (2021-2022)
5. Ayush Das (2022)